BIOCHEMISTRY — VITAMINS

💫 Fat-Soluble Vitamins

A, D, E, K — the ones that hide in your fat, build up to toxic levels, and show up on every board exam.

A patient on isotretinoin develops headache, papilledema, and enlarged ventricles on CT. What vitamin is responsible?

The Big Picture

Why "Fat-Soluble" Matters

It's not just a classification — it changes how they hurt you

A, D, E, and K dissolve in fat, not water. They accumulate in adipose tissueFat cells act as a reservoir. The vitamin slowly leaches out — this is why toxicity can develop insidiously and persist even after you stop taking supplements.. This means two things boards love to test: they can reach toxic levels (unlike most water-soluble vitamins), and deficiency takes longer to develop because the body has reserves.
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Board trap: If Ca and PO4 move in opposite directions → think PTH (Vitamin A connection). If they move in the same direction → think Vitamin D.

Vitamin Dossiers

The Fat Four

Tap each section to reveal clinical details

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Vitamin A
Retinol • Retinoic Acid
Key rolesCofactor for PTHParathyroid hormone — stimulates osteoclasts, raises serum Ca, lowers PO4. When PTH is involved, Ca and PO4 always go in opposite directions. function • Epithelial cell maturation (hair, skin, eyes) • Night vision • CSF production • Mild antioxidant
UniqueNight vision is the most board-testable function
Night blindness (earliest sign) • Dry eyes (xerophthalmiaStarts with Bitot spots (foamy white patches on conjunctiva), progresses to corneal ulceration and blindness if untreated.) • Dry skin, poor wound healing • Immune dysfunction

Clinical uses: Give Vitamin A in measles (regenerates lung epithelium), burn patients, cancer (retinoids T15/17), any infection destroying epithelial cells.
Pseudotumor cerebri (idiopathic intracranial hypertension):
• Headache + papilledemaSwelling of the optic disc from increased intracranial pressure. Look for it with a fundoscopic exam. Main complication is blindness.
• CT shows dilated ventricles
• This is the only cause of increased ICP where you don't worry about herniation
• Also see: hyperparathyroidism ("moans, groans, bones, and stones")

Management: D/C vitamin A → serial lumbar punctures (remove ≤30cc/24hrs to avoid osmotic demyelinationCSF is continuous with plasma. Removing too much too fast causes osmotic shifts that damage the pons (central pontine myelinolysis).) → Chronic: weight loss + acetazolamide

Most common cause of pseudotumor cerebri: obesity. #2: Vitamin A toxicity.
Main complication: blindness.
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Vitamin D
Cholecalciferol • Calcitriol (active)
Key rolesCalcium absorptionVitamin D upregulates calcium-binding proteins (calbindin) in the intestinal epithelium, allowing Ca to be absorbed from food into the bloodstream. from the gut • Bone mineralization • Phosphorus regulation
UniqueCa and PO4 move in the same direction (both up or both down)
ActivationSkin (UV) → Liver (25-hydroxylation) → KidneyThe kidney performs the final 1α-hydroxylation step, converting 25-OH-D to active 1,25-(OH)₂-D (calcitriol). This is why chronic kidney disease causes Vitamin D deficiency. (1α-hydroxylation) → Active calcitriol
Children: Ricketsbowed legs, frontal bossing, rachitic rosary (beading at costochondral junctions), craniotabes (soft skull)

Adults: Osteomalacia — soft bones, bone pain, fractures, proximal muscle weakness

Lab findings: ↓Ca, ↓PO4, ↑PTH (secondary hyperparathyroidism), ↑alkaline phosphatase

At-risk: CKD patients (can't do 1α-hydroxylation), dark skin + northern climates, malabsorption (Crohn's, celiac), elderly homebound
Hypercalcemia: "stones, bones, groans, and psychiatric overtones"
• Kidney stones, bone pain, constipation, confusion
Metastatic calcificationWhen calcium levels get high enough, it starts depositing in soft tissues — blood vessels, kidneys, lungs. Different from dystrophic calcification which happens in damaged tissue regardless of Ca levels. — calcium deposits in soft tissues
• Polyuria (calcium interferes with ADH → nephrogenic DI)
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Vitamin E
Tocopherol • The Bodyguard
Key rolesMajor antioxidant — protects cell membranes from free radicalHigh-energy oxygen molecules that pierce through cell membranes and nuclear membranes to damage DNA. Cells then either die or mutate (cancer). Most common source: infections (especially viruses). Neutrophils make them via NADPH oxidase. damage • Works synergistically with selenium
UniqueThe major antioxidant (Vitamin A is only a "mild" antioxidant)
Without membrane protection:
Hemolytic anemia (RBC membranes burst — especially in premature newborns)
• Neurological deficits: posterior column and spinocerebellar tract degeneration (looks like B12 deficiency neurologically)
• Ataxia, loss of proprioception

At-risk: Premature infants, fat malabsorption syndromes (cystic fibrosis, celiac, abetalipoproteinemia)
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Vitamin K
Phylloquinone (K1) • Menaquinone (K2)
Key rolesGamma-carboxylationAdds a carboxyl group to glutamic acid residues on clotting factors, allowing them to bind calcium and become active. Without this step, the factors are produced but non-functional. of clotting factors 2, 7, 9, 10 + Protein C & S
SourceGut flora (K2) + leafy greens (K1)
UniqueThe clotting vitamin — warfarin is a Vitamin K antagonist
Increased PT/INR (factor 7 has the shortest half-life, so PT rises first)
• Easy bruising, mucosal bleeding, hemorrhage

At-risk populations:
Newborns — sterile gut, no flora to make K2. This is why every newborn gets a Vitamin K injection at birth
• Patients on prolonged antibiotics (kill gut flora)
• Fat malabsorption
• Warfarin use (by design)

Reversal: Give Vitamin K (takes hours) or FFPFresh Frozen Plasma provides the actual clotting factors immediately. Use for acute/life-threatening bleeds. Vitamin K takes 6-24 hours to work because the liver needs time to synthesize new functional factors. (immediate, for emergencies)

Challenge

Sort the Vitamins

Drag each vitamin to the correct category

Vitamin A
Vitamin C
Vitamin D
Folate (B9)
Vitamin E
Thiamine (B1)
Vitamin K
Niacin (B3)
B12
🌎 Fat-Soluble
💧 Water-Soluble

High-Yield Comparison

Calcium & Phosphorus Direction

This distinction shows up on every form of boards

ConditionCaPO4DirectionThink...
PTH ↑ (Vitamin A excess, primary hyperPTH)OppositePTH
Vitamin D excessSameVitamin D
Vitamin D deficiencySameVitamin D
PseudohypoparathyroidismOppositePTH resistance
CKD (no 1α-hydroxylation)OppositeSecondary hyperPTH + phosphate retention
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The rule: Calcium and the hormone (PTH) always go in the same direction. If Ca and PO4 go in the same direction, the problem is Vitamin D, not PTH.

Quiz Time

Test Yourself

10 questions, randomized from a pool of 15

Correct answers